치주인대세포에서 기계적 힘에 의한 Secretory Leukocyte Peptidase Inhibitor 발현과 기능
Role of Mechanical Force-Induced Secretory Leukocyte Peptidase Inhibitor in Periodontal Ligament Cells
유홍일, 김현수, 양동욱, 이수영, 김선헌,
소속 상세정보
유홍일 ( Yoo Hong-Il ) - Eulji University College of Medicine Department of Anatomy and Neuroscience
김현수 ( Kim Hyeon-Su ) - Chonnam National University School of Dentistry Department of Oral Anatomy
양동욱 ( Yang Dong-Wook ) - Chonnam National University School of Dentistry Department of Oral Anatomy
이수영 ( Lee Su-Young ) - Chonnam National University School of Dentistry Department of Oral Anatomy
김선헌 ( Kim Sun-Hun ) - Chonnam National University School of Dentistry Department of Oral Anatomy
KMID : 0355820220430010001
Abstract
Multiple genes are involved in periodontal tissue remodeling in orthodontic tooth movement (OTM). Studies have attempted to establish the precise mechanism of remodeling during OTM. This study investigated the mechanism of action and functional roles of secretory leukocyte peptidase inhibitor (SLPI), a molecule enhancing tissue repair during OTM. The upper first molars of rats were moved mesially by applying a Ni-Ti coil spring on the upper incisors as an anchor. Primary cultures were prepared from human periodontal ligament (HPDL) cells and subjected to tension and compression forces. In vivo: The SLPI level increased at 1d, 2d, and 6d after OTM by real time-PCR and Western blot. Immunoreactivity against SLPI was mainly found in the PDL and enhanced in the tension and compression side. In vitro: SLPI worked by acting on MAPK pathways including ERK and p38. SLPI mRNA was upregulated by tension or compression. SLPI siRNA treatment induced upregulation of IL-6 and IL-8 in HPDL cells during the tension applications. However, SLPI siRNA treatment induced the downregulation of IL-8 during compression application. These results suggest that SLPI in the PDL may be involved in inflammation control in OTM.
키워드
치주인대; 기계적 힘; SLPI; 치아교정
PDL; mechanical force; SLPI; orthodontics
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